第58届Intel ISEF中国参赛项目：肝细胞生长因子对视网膜色素上皮光损伤的保护作用
东北育才学校 高三 李晨
学科：医学与健康
为探讨肝细胞生长因子对视网膜色素上皮光损伤的保护作用，采用逆转录PCR从肝组织中得到人肝细胞生长因子（hHGF）基因，并将该基因克隆到载体pcDNA3.1（＋）中，构建重组真核表达载体pcDNA－hHGF，用脂质体lipofectamine将载体pcDNA－hHGF转染体外培养的人视网膜色素上皮（RPE）细胞，并用ELISA检测RPE细胞培养液中的hHGF，然后将转基因的RPE细胞和非转基因的RPE细胞进行光照，用单细胞凝胶电泳方法检测RPE细胞所受到的损伤，比较两组细胞损伤的差异。单细胞凝胶电泳结果表明，转hHGF基因的视网膜色素上皮细胞实验组与非转基因的视网膜色素上皮细胞对照组相比，细胞彗星尾长及尾/头明显变小，其差异具有显著统计学意义(P<0.01)。证实了肝细胞生长因子对视网膜色素上皮细胞的光损伤具有一定的保护作用。
The Protective Effect of HGF against The Light Damage to Retinal Pigment Epithelium
Medcine & Health Sciences
Li Chen 19, Northeast YuCai School
To investigate HGF-mediated protective effect against the light damage to retinal pigment epithelium cells( hRPE ). The cDNA of hHGF was obtained from liver by RT-PCR and inserted into plasmid vecter pcDNA3.1. Then the vecter of pcDNA-hHGF was constructed. The constructed plasmid was transfected into hRPE cells cultured in vitro by a lipfection technology. The expression of hHGF was examined by ELISA technology. Then transfected cultured RPE cells and nontrasfected cultured RPE cells were treated with constant exposure to light.The damage level of both groups was examined by the single cell gel electrophoresis. The result indicats that hHGF transfected hRPE cells significantly survive than nontrasfected hRPE cells. This investigation demostrats that hHGF has significant protective effect against the light damage to retinal pigment epithelium cells.
